Latoscha#, A., Drexler#, D.J., Al-Bassam, M.M., Kaever, V., Findlay, K.C., Witte*, G., and N. Tschowri* (2019) c-di-AMP hydrolysis by a novel type of phosphodiesterase promotes differentiation of multicellular bacteria. bioRxiv preprint first posted online Oct. 1;
(# these authors contributed equally to this work, * corresponding authors) doi: http://dx.doi.org/10.1101/789354.

Latoscha,A., Wörmann, M.E., and N. Tschowri (2019) Nucleotide second messengers in Streptomyces. Microbiology. Sep 19. doi: 10.1099/mic.0.000846. [Epub ahead of print]
» PubMed 

Gibhardt, J., Hoffmann, G., Turdiev, A., Wang, M., Lee, V.T., and F. M. Commichau (2019)
c-di-AMP assists osmoadaptation by regulating the Listeria monocytogenes potassium transporters KimA and KtrCD. J Biol Chem. Sep 9. pii: jbc.RA119.010046.
doi: 10.1074/jbc.RA119.010046. [Epub ahead of print
] » PubMed 

Conradi, F.D., Zhou, R.Q., Oeser, S., Schuergers, N., Wilde, A., and C.W. Mullineaux (2019) Factors controlling floc formation and structure in the cyanobacterium Synechocystis sp. PCC 6803. J Bacteriol. Sep 6;201(19). pii: e00344-19. doi: 10.1128/JB.00344-19. Print 2019 Oct 1. » PubMed 

McLean, T.C., Lo, R., Tschowri, N., Hoskisson, P.A., Al Bassam, M.M., Hutchings, M.I., and N.F. Som (2019) Sensing and responding to diverse extracellular signals: an updated analysis of the sensor kinases and response regulators of Streptomyces species. Microbiology. Sep;165(9):929-952. doi: 10.1099/mic.0.000817. » PubMed 

Schäfer, H., and K. Turgay (2019) Spx, a versatile regulator of the Bacillus subtilis stress response. Curr Genet. Aug;65(4):871-876. doi: 10.1007/s00294-019-00950-6. Epub 2019 Mar 4. » PubMed 

Heidemann, J.L., Neumann, P., Dickmanns, A., and Ralf Ficner (2019) Crystal structures of the c-di-AMP synthesizing enzyme CdaA. J Biol Chem. Jul 5;294(27):10463-10470. doi: 10.1074/jbc.RA119.009246. Epub 2019 May 22. » PubMed 

Serra, D.O., and R. Hengge (2019) Cellulose in Bacterial Biofilms. In: Cohen E., Merzendorfer H. (eds) Extracellular Sugar-Based Biopolymers Matrices. Biologically-Inspired Systems, vol 12.
pp 355-392. Springer, Cham.
» https://link.sprinhttps://link.springer.com/chapter/10.1007/978-3-030-12919-4_8

Schäfer, H.,  Beckert, B., Steinchen, W., Nuss, A., Beckstette, M., Hantke, I., Sudzinová, P., Krásný, L., Kaever, V., Dersch, P., Bange, G.,  Wilson, D.,  and K. Turgay (2019) The alarmone (p)ppGpp is part of the heat shock response of Bacillus subtilis. bioRxiv preprint first posted online Jul. 1. doi: http://dx.doi.org/10.1101/688689

R. Hengge (2019) Targeting Bacterial Biofilms by the Green Tea Polyphenol EGCG. Molecules. Jun 29;24(13). pii: E2403. doi: 10.3390/molecules24132403. » PubMed 

Bense, S., Bruchmann, S., Steffen, A., Stradal, T.E.B., Häussler*, S., and J. Düvel* (2019) Spatiotemporal control of FlgZ activity impacts Pseudomonas aeruginosa flagellar motility. Mol Microbiol. Jun;111(6):1544-1557. doi: 10.1111/mmi.14236. Epub 2019 Mar 29.
(* corresponding authors) » PubMed

Gundlach, J., Krüger, L., Herzberg, C., Turdiev, A., Poehlein, A., Tascón, I. , Weiß, M. , Hertel, D., Daniel, R, Hänelt, I., Lee, V.T., and J. Stülke (2019) Sustained sensing in potassium homeostasis: Cyclic di-AMP controls potassium uptake by KimA at the levels of expression and activity. J Biol Chem. Jun 14;294(24):9605-9614. doi: 10.1074/jbc.RA119.008774. Epub 2019 May 6. » PubMed 

Pfiffer, V., Sarenko, O., Possling, A., and R. Hengge (2019) Genetic dissection of Escherichia coli’s master diguanylate cyclase DgcE: Role of the N-terminal MASE1 domain and direct signal input from a GTPase partner system. PLoS Genet. Apr 25;15(4):e1008059.
doi: 10.1371/journal.pgen.1008059. eCollection 2019 Apr. » PubMed 

Quintana, I.M., Gibhardt, J., Turdiev, A., Hammer, E., Commichau, F.M., Lee, V.T., Magni*, C. and Jörg Stülke* (2019) The KupA and KupB proteins of Lactococcus lactis IL1403 are novel c-di-AMP receptor proteins responsible for potassium uptake. J. Bacteriol. Apr 24;201(10). pii: e00028-19. doi: 10.1128/JB.00028-19. Print 2019 May 15. (* corresponding authors)
» PubMed
Highlighted in the J. Bacteriol. Journal:
Pham. H.T. and Turner, M.S: :
Onwards and [K+]upwards: a new potassium importer under the spell of cyclic-di-AMP. J Bacteriol. 2019 Apr 24;201(10). pii: e00150-19. doi: 10.1128/JB.00150-19. Print 2019 May 15. » PubMed

Serra, D.O., and R. Hengge (2019) A c-di-GMP-Based Switch Controls Local Heterogeneity of Extracellular Matrix Synthesis which Is Crucial for Integrity and Morphogenesis of Escherichia coli Macrocolony Biofilms. J Mol Biol. Apr 4. pii: S0022-2836(19)30177-9.
doi: 10.1016/j.jmb.2019.04.001. [Epub ahead of print]
» PubMed 

Rossmann#, F.M., Rick#, T., Mrusek, D., Sprankel, L., Dörrich, A.K., Leonhard, T., Bubendorfer, S., Kaever, V., Bange, G., and K.M. Thormann (2019) The GGDEF domain of the phosphodiesterase PdeB in Shewanella putrefaciens mediates recruitment by the polar landmark protein HubP. J. Bacteriol. Mar 13;201(7). pii: e00534-18. doi: 10.1128/JB.00534-18. Print 2019 Apr 1. (# equal contribution) » PubMed 
Highlighted in the J. Bacteriol. Journal:
Articles of Significant Interest Selected by the Editors: Exploring How a Phosphodiesterase Is Recruited to the Cell Pole in Shewanella putrefaciens
» https://jb.asm.org/content/201/7/e00100-19

Hengge, H., Häussler, S., Pruteanu, M., Stülke, J., Tschowri, N., and K. Turgay (2019) Recent Advances and Current Trends in Nucleotide Second Messenger Signaling in Bacteria.
J Mol Biol. Mar 1;431(5):908-927. doi:10.1016/j.jmb.2019.01.014. Epub 2019 Jan 19.
» PubMed 

Schäfer, H., Heinz, A., Sudzinová, P., Voß, M., Hantke, I., Krásný, L., and K. Turgay (2019)
Spx, the central regulator of the heat- and oxidative stress response in B. subtilis, can repress transcription of translation-related genes. Mol Microbiol Feb;111(2):514-533. doi: 10.1111/mmi.14171. Epub 2018 Dec 11. » PubMed 

Hantke, I., Schäfer, H., Janczikowski, A., and K. Turgay (2019) YocM a small heat shock protein can protect Bacillus subtilis cells during salt stress. Mol Microbiol. Feb;111(2):423-440. doi: 10.1111/mmi.14164. Epub 2018 Dec 4» PubMed 

Thomanek, N., Arends, J., Lindemann, C., Barkovits, K., Meyer, H.E., Marcus, K.,  and N. Narberhaus (2019) Intricate crosstalk between lipopolysaccharide, phospholipid and fatty acid metabolism in Escherichia coli modulates proteolysis of LpxC. Front. Microbiol. Jan 14;9:3285. doi: 10.3389/fmicb.2018.03285. eCollection 2018. » PubMed


Pausch, P., Steinchen, W., Wieland, M., Klaus, T., Freibert, S.A., Altegoer, F., Wilson, D.N., and G. Bange (2018) Structural basis for (p)ppGpp-mediated inhibition of the GTPase RbgA.
J Biol Chem Dec 21;293(51):19699-19709. doi: 10.1074/jbc.RA118.003070. Epub 2018 Oct 26. » PubMed 

Commichau, F.M., Heidemann, J.L., Ficner, R., and J. Stülke (2018) Making and breaking of an essential poison: the cyclases and phosphodiesterases that produce and degrade the essential second messenger cyclic di-AMP in bacteria. J Bacteriol. Dec 7;201(1). pii: e00462-18. doi: 10.1128/JB.00462-18. Print 2019 Jan 1. » PubMed 

Bedrunka, P.,  Olbrisch, F.,  Rüger, M.,  Zehner, S., and N. Frankenberg-Dinkel (2018) Nitric oxide controls c-di-GMP turnover in Dinoroseobacter shibae. Microbiology. Nov;164(11):1405-1415. doi: 10.1099/mic.0.000714. Epub 2018 Sep 17» PubMed 

Al-Bassam, Haist#, J., Neumann#, S.A., Lindenberg, S., and N. Tschowri (2018)
Expression patterns, genomic conservation and input into developmental regulation of the GGDEF/EAL/HD-GYP domain proteins in Streptomyces. Front. Microbiol., Oct 23;9:2524.
doi: 10.3389/fmicb.2018.02524. eCollection 2018. (# equal contribution) » PubMed 

Beckert# B., Turk#, M., Czech, A., Berninghausen, O., Beckmann, R., Ignatova, Z., Plitzko*, J.M., and D.N. Wilson* (2018) Structure of a hibernating 100S ribosome reveals an inactive conformation of the ribosomal protein S1. Nat Microbiol Oct;3(10):1115-1121. doi: 10.1038/s41564-018-0237-0. Epub 2018 Sep 3. (# equal contribution, * corresponding authors) » PubMed 
Journal cover October 2018
Highlighted in:
1. Informationsdienst Wissenschaft: Forschungsteam der Universität Hamburg identifiziert Aufbau von 100S-Ribosomen » Read more
2. FOCUS Online: Universität Hamburg – Meldung vom 04.09.2018 » Read more
3. LABO Online: Aufbau von 100S-Ribosomen identifiziert » Read more
4. LaborPraxis: Beim Rendezvous der Ribosomen zugeschaut » Read more

Keller, H., Weickhmann, A.K., Bock, T., and J. Wohnert (2018) Adenine protonation enables cyclic-di-GMP binding to cyclic-GAMP sensing riboswitches. RNA. Oct;24(10):1390-1402. doi: 10.1261/rna.067470.118. Epub 2018 Jul 13. » PubMed 

Quintana, I., Espariz, M., Villar, S., Gonzalez, F. B., Pacini, F., Cabrera, G., Bontempi, I., Procheto, E., Stülke, J., Perez, A. R., Marcipar, I., Blancato, V., and C. Magni (2018) Genetic engineering of Lactococcus lactis co-producing antigen and the mucosal adjuvant 3′ 5′- cyclic di adenosine monophosphate (c-di-AMP) as a design strategy to develop a mucosal vaccine prototype. Front. Microbiol. Sep 4;9:2100. doi: 10.3389/fmicb.2018.02100. eCollection 2018.
» PubMed 

Klauck, G., Serra, D.O., Possling, A., and R. Hengge (2018) Spatial organization of different sigma factor activities and c-di-GMP signalling within the three-dimensional landscape of a bacterial biofilm. Open Biol. Aug 8(8). pii: 180066. doi: 10.1098/rsob.180066» PubMed 

Gratani, F.L., Horvatek, P., Geiger, T., Borisova, M., Mayer, C., Grin, I., Wagner, S., Steinchen, W., Bange, G., Velic, A., Maček, B., and C. Wolz (2018)
Regulation of the opposing (p)ppGpp synthetase and hydrolase activities in a bifunctional RelA/SpoT homologue from Staphylococcus aureusPLoS Genet. Jul 9;14(7):e1007514. doi: 10.1371/journal.pgen.1007514. eCollection 2018 Jul» PubMed 

Commichau, F.M., and J. Stülke (2018) Coping with an essential poison: a genetic suppressor analysis corroborates a key function of c-di-AMP in controlling potassium ion homeostasis in Gram-positive bacteria. J Bacteriol. May 24;200(12). pii: e00166-18. doi: 10.1128/JB.00166-18. Print 2018 Jun 15» PubMed

Herbst, S., Lorkowski, M., Sarenko, O., Nguyen, T.K.L., Jaenicke, T., and R. Hengge (2018)
Transmembrane redox control and proteolysis of PdeC, a novel type of c-di-GMP
phosphodiesterase. EMBO J. Apr 13;37(8). pii: e97825. doi: 10.15252/embj.201797825. Epub 2018 Mar 7. » PubMed 

Commichau, F.M., Gibhardt, J., Halbedel, S., Gundlach, J., and J. Stülke (2018) A Delicate Connection: c-di-AMP Affects Cell Integrity by Controlling Osmolyte Transport. Trends Microbiol. Mar;26(3):175-185. doi: 10.1016/j.tim.2017.09.003. Epub 2017 Sep 28.
» PubMed
Journal cover March 2018
Highlighted by Faculty of 1000:
Waters, C. : In F1000Prime, 09 October 2017. doi: 10.3410/f.731603148.793537473. 

Gundlach, J., Commichau, F.M., and J. Stülke (2018) Of ions and messengers: an intricate link between potassium, glutamate, and cyclic di-AMP. Curr Genet. Feb;64(1):191-195. doi: 10.1007/s00294-017-0734-3. Epub 2017 Aug 20. » PubMed

Thongsomboon, W., Serra, D.O., Possling, A., Hadjineophytou, C.Hengge*, R., and
L. Cegelski* 
(2018) Phosphoethanolamine cellulose: A naturally produced chemically modified cellulose. Science. Jan 19;359(6373):334-338. doi: 10.1126/science.aao4096. (*corresponding authors) » PubMed 
Highlighted in:
1. Perspectives Section of the Science Journal: Galperin, M. and Shalaeva, D.: A bacterial coat that is not pure cotton. Science 2018 Jan 19;359(6373):276-277. doi: 10.1126/science.aar5253» Read more
2. Science News: The secret to icky, sticky bacterial biofilms lies in the microbes’ cellulose. » Read more
3. Nature highlight: How bacteria make their fortresses. » Read more
4. Chemical & Engineering News: Bacteria make modified cellulose. » Read more
5. Phys.org News: Bacterial biofilm cellulose found to differ from plant cellulose. » Read more
6. Nature Microbiology: Do Gram-negatives produce cellulose or not? » Read more
7. F1000Prime by G. Nester, 12 February 2018. doi: 10.3410/f.732501572.793542371.
F1000Prime by C. Whitfield, 16 February 2018. doi: 10.3410/f.732501572.793542459.


Drexler, D.J., Müller, M., Rojas-Cordova, C.A., Bandera, A.M., and G. Witte (2017) Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritimaStructure. Oct 24. pii: S0969-2126(17)30329-5. doi: 10.1016/j.str.2017.10.001. Epub 2017 Oct 26. » PubMed

Sarenko, O., Klauck, G., Wilke, F.M., Pfiffer, V., Richter,A.M., Herbst, S., Kaever, V., and R. Hengge (2017) More than enzymes that make and break c-di-GMP – Local signaling in the interactome of GGDEF/EAL domain proteins of Escherichia coli. mBio. Oct 10;8(5). pii: e01639-17. doi: 10.1128/mBio.01639-17. » PubMed
Highlighted by Faculty of 1000:
1. Sondermann, H. : In F1000Prime, 19 October 2017. doi: 10.3410/f.731966577.793537737;
2. Galperin, M. : In F1000Prime, 20 October 2017. doi:10.3410/f. 731966577.793537754.

Bähre, H., and V. Kaever (2017) Identification and Quantification of Cyclic Di-Guanosine
Monophosphate and Its LinearMetabolites by Reversed-Phase LC-MS/MS. In: Sauer K. (eds)
c-di-GMP Signaling. Methods in Molecular Biology, vol 1657. Humana Press, New York, NY, 1657:45-58. doi: 10.1007/978-1-4939-7240-1_5. » PubMed

Serra, D.O., and R. Hengge (2017) Experimental detection and visualization of the extracellular matrix in macrocolony biofilms. In: Sauer K. (eds) c-di-GMP Signaling. Methods in Molecular Biology, vol 1657. Humana Press, New York, N.Y., 1657:133-145. doi: 10.1007/978-1-4939-7240-1_11. » PubMed

Angerer, V., Essen, L.O., and A. Wilde (2017) Analysis of c-di-GMP levels synthesized by a photoreceptor protein in response to different light qualities using an in vitro enzymatic assay. In: Sauer K. (eds) c-di-GMP Signaling. Methods in Molecular Biology, vol 1657. Humana Press, New York, N.Y., 1657:187-204. doi: 10.1007/978-1-4939-7240-1_15. » PubMed

Kampf, J., Gundlach, J., Herzberg, C., Treffon, K.,  and J. Stülke (2017) Identification of c-di-AMP-binding proteins using magnetic beads. In: Sauer K. (eds) c-di-GMP Signaling. Methods in Molecular Biology, vol 1657. Humana Press, New York, N.Y., 1657:347-359. doi: 10.1007/978-1-4939-7240-1_27» PubMed

Beckert#, B., Abdelshahid#, M., Schäfer, H., Steinchen, W., Arenz, S., Berninghausen, O., Beckmann, R., Bange*, G., Turgay*, K., and D.N. Wilson* (2017) Structure of the Bacillus subtilis hibernating 100S ribosome reveals the basis for 70S dimerization. EMBO J. Jul 14;36(14):2061-2072. doi: 10.15252/embj.201696189. Epub 2017 May 3. (# equal contribution, * corresponding authors) » PubMed
Highlighted in the News & Views section of the EMBO Journal:
Gonzalez, R.L. Jr. : Survivor: Ribosome Edition. EMBO J. 2017 Jul 14;36(14):1996-1998. doi: 10.15252/embj.201797518. » PubMed

Blötz, C., Treffon, K., Kaever, V., Schwede, F., Hammer, E., and J. Stülke (2017) Identification of the components involved in cyclic di-AMP signaling in Mycoplasma pneumoniae. Front. Microbiol. Jul 13;8:1328. doi: 10.3389/fmicb.2017.01328eCollection 2017. » PubMed

Gundlach, J., Herzberg, C., Hertel, D., Thürmer, A., Daniel, R., Link, H., and J. Stülke (2017) Adaptation of Bacillus subtilis to life at extreme potassium limitation. mBio. Jul 5;8(4). pii: e00861-17. doi: 10.1128/mBio.00861-17» PubMed
Highlighted by Faculty of 1000:
Clausen, T. and Suskiewicz, M. : In F1000Prime, 06 October 2017. doi: 10.3410/f.727781851.793537409.

Angerer, V., Schwenk, P., Wallner, T., Kaever, V., Hiltbrunner, A., and A. Wilde (2017) The protein Slr1143 is an active diguanylate cyclase in Synechocystis sp. PCC 6803 and interacts with the photoreceptor Cph2. Microbiology. Jun;163(6):920-930. doi: 10.1099/mic.0.000475. Epub 2017 Jun 21. » PubMed

Schumacher, MA., Zeng, W., Findlay, K.C., Buttner, M.J., Brennan, R.G., and N. Tschowri (2017) The Streptomyces master regulator BldD binds c-di-GMP sequentially to create a functional BldD2-(c-di-GMP)4 complex. Nucleic Acids Res. Jun 20;45(11):6923-6933. doi: 10.1093/nar/gkx287. » PubMed

Schuergers, N., Mullineaux, C.W., and Wilde, A. (2017) Cyanobacteria in motion. Curr. Opin. Plant Biol. Jun;37:109-115. doi: 10.1016/j.pbi.2017.03.018Epub 2017 May 1. » PubMed

Gundlach, J., Herzberg, C., Kaever, V., Gunka, K., Hoffmann, T., Weiß, M., Gibhardt, J., Thürmer, A., Hertel, D., Daniel, R., Bremer, E., Commichau, F.M., and J. Stülke (2017) Control of potassium homeostasis is an essential function of the second messenger cyclic di-AMP in Bacillus subtilis. Sci. Signal. Apr 18;10(475). pii: eaal3011. doi: 10.1126/scisignal.aal3011.
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Krol, E., Klaner, C., Gnau, P., Kaever, V., Essen, LO., and A. Becker (2016) Cyclic mononucleotide- and Clr-dependent gene regulation in Sinorhizobium meliloti. Microbiology. Oct;162(10):1840-1856. doi: 10.1099/mic.0.000356. Epub 2016 Aug 16. » PubMed

Arenz, S., Abdelshahid, M., Sohmen, D., Payoe, R., Starosta, A.L., Berninghausen, O., Hauryliuk, V., Beckmann, R., and D.N. Wilson (2016) The stringent factor RelA adopts an open conformation on the ribosome to stimulate ppGpp synthesis. Nucleic Acids Res. Jul 27;44(13):6471-81. doi: 10.1093/nar/gkw470. Epub 2016 May 25. » PubMed